John Rubinstein

Canada Research Chair in Electron Cryomicroscopy

Tier 1 - 2017-11-01
Renewed: 2021-04-01
University of Toronto
Canadian Institutes of Health Research

Research involves

Investigating the 3D structures that make up cells, and using the resulting technology to better understand a class of enzymes called ATPases.

Research relevance

This research could lead to a better understanding of how use a class of enzymes called rotary ATPases to treat diseases like cancer and osteoporosis.

Research summary

Electron cryomicroscopy (cryo-EM) is a technique in structural biology that uses electrons to study samples that have been frozen at cryogenic temperatures. It can help researchers look inside cells to understand how they work at the molecular level. As Canada Research Chair in Electron Cryomicroscopy, Dr. John Rubinstein aims to develop tools to expand the capabilities of cryo-EM.

Rubinstein and his research team are using cryo-EM to better understand the control and flow of energy in biological systems. For example, they are studying mitochondrial respiration to better understand how this process changes in cancer, mitochondrial disease and neurodegeneration. They are also investigating mycobacterial respiration to support the development of new treatments for tuberculosis and other mycobacterial infections. In a related project, they are looking for new treatments for osteoporosis, cancer and neurological diseases.